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KMID : 0848120170420040149
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International Journal of Oral Biology
2017 Volume.42 No. 4 p.149 ~ p.153
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Induction of Prostaglandin E2 by Porphyromonas gingivalis in Human Dental Pulp Cells
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Kim So-Hee
Paek Yun-Woong
Kang In-Chol
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Abstract
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Cyclooxygenase-2 (COX-2)-mediated prostaglandin E2 (PGE2) plays a key role in development and progression of inflammatory responses and Porphyromonas gingivalis is a common endodontic pathogen. In this study, we investigated induction of COX-2 and PGE2 by P. gingivalis in human dental pulp cells (HDPCs). P. gingivalis increased expression of COX-2, but not that of COX-1. Increased levels of PGE2 were released from P. gingivalis-infected HDPCs and this PGE2 increase was blocked by celecoxib, a selective COX-2 inhibitor. P. gingivalis activated all three types of mitogen-activated protein kinases (MAPKs). P. gingivalis-induced activation of nuclear factor-κB (NF-κB) was demonstrated by the results of phosphorylation of NF-κ B p65 and degradation of inhibitor of κB-α (IκB-α). Pharmacological inhibition of each of the three types of MAPKs and NF-κB substantially attenuated P. gingivalisinduced PGE2 production. These results suggest that P. gingivalis should promote endodontic inflammation by stimulating dental pulp cells to produce PGE2.
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KEYWORD
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Human dental pulp cells, prostaglandin E2, cyclooxygenase-2, Porphyromonas gingivalis
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